Part:BBa_K2665014:Design

TDH3 promoter->SseNHX1->6xHis->CYC terminator

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 2379
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We added promoter and terminator in order to express the ORF, and added His-tag for Western Blotting.

Source

The ORF is created by DNA shuffring using 2 kinds of plant.